Abstract

Abstract Introduction Blood is a biological material with high potential of infectious transmission in dental environments, including herpes simplex, hepatitis and AIDS. Aim To investigate the efficacy of luminol in detecting blood in endodontic files before and after the sterilization process. Material and method Luminol was used to investigate the presence or absence of traces of blood tissue in 50 endodontic files, visible to naked eye or not, after performing endodontic treatment and after the cleaning/sterilization process. The results obtained were tabulated and statistically analyzed by using the Friedman’s test at a significance level of 5% (p<0.05). Result By naked eye, it was found that 31/50 files showed no trace of blood, 8/50 showed a slight presence of blood and 11/50 showed a considerable presence of blood after endodontic treatment. After the use of luminol, however, 16/50 endodontic files showed no trace of blood, 19/50 showed a slight presence of blood and 15/50 showed a considerable presence of blood. After the cleaning and sterilization process, no blood was detected in the files. Conclusion It was concluded that the luminol solution is effective in detecting blood tissue in endodontic files as well as in validating the cleaning/sterilization process.

Highlights

  • Blood is a biological material with high potential of infectious transmission in dental environments, including herpes simplex, hepatitis and AIDS

  • Major infectious diseases related to the dental surgery practice are herpes simplex, Arruda-Vasconcelos, Chantre, Lopes et al Rev Odontol UNESP. 2017 July-Aug; 46(4): 227-231 hepatitis, and AIDS

  • This study has evaluated endodontic files used during endodontic treatment performed by final-year undergraduate students

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Summary

Introduction

Blood is a biological material with high potential of infectious transmission in dental environments, including herpes simplex, hepatitis and AIDS. Material and method: Luminol was used to investigate the presence or absence of traces of blood tissue in 50 endodontic files, visible to naked eye or not, after performing endodontic treatment and after the cleaning/sterilization process. Cross-infection control will always be an important area of concern as new and emerging pathogens have been frequently isolated and drug resistance has been increasing as well. This forms an important part of practice for all healthcare professionals and remains one of the most cost-effective medical interventions available[2]. Infections caused by viruses are the most severe and of most concern when contracted[3,4]

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