Abstract
A new high-performance liquid chromatography (HPLC)–electron spin resonance (ESR) spin-trapping system for post-column on-line detection of the superoxide radical scavenging activity of column eluates was developed. A cyclic nitrone-type spin-trapping reagent, 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), was used for detection of the superoxide radical, which was produced by riboflavin-dependent photo-reduction of molecular oxygen in the presence of an electron donor. The reaction conditions were optimized by monitoring the ESR signal intensity of the spin-adduct of DMPO. HPLC–ESR chromatographs were recorded for the standard sample solution, which included three phenolic compounds, gallic acid (Gal), 4-hydroxycoumaric acid (Cou), and caffeic acid (Caf).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
