Abstract

Objective To establish and validate a standard method using flow cytometry （FCM）in human ABO blood group antibody （Ab） detection. Methods Sera samples from 52 blood donors were incubated with standardize red blood cells （RBCs）, and anti-A/B antibody （IgM/IgG） was measured by indirect flow cytometry after adding secondary isotype-specific fluoresce labeled Ab. The results were compared with those by using ELISA reader. Results The changes of anti-A/B IgM measured by both methods correlated well and an overall correlation coefficiency of 0.730 for IgM was obtained by Spearman＇s rank testing （P〈0.05）. FCM showed better specificity, sensitivity and reproducibility over ELISA reader. In blood group A samples （n = 16） or B samples （n = 16） ,the antirespectively. IgG blood group Ab could only be detected in blood group O sera. Conclusion Compared with traditional methods,FCM is a more objective method to offer accurate detection of natural ABO blood group Ab （IgM/IgG） and allowed semi-quantitative measurement of antibody levels. Key words: Flow cytometry; ABO blood-group system

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