Application of fast light-readapted plants for measurement of chlorophyll fluorescence and P<sub>700</sub> light absorption with the RLC method

Abstract

The rapid light curve method is a time-saving approach to the measurement of photosynthetic processes over a wide spectrum of light intensities. The adaptation to darkness enables the calculation of a complete set of coefficients for chlorophyll (Chl) fluorescence and P700 light absorption; however, dark-adapted plants demonstrate drastically different patterns of Chl fluorescence than that of light-adapted plants. The present work compared light-adapted barley plants without dark adaptation (L), and barley plants adapted to darkness and readapted to light for 7.5 min (D/L). The rapid light curves were generally similar in both the L and D/L variants. Some differences between the L and D/L variants were found in plants grown at any illumination. A number of distinctions between the L and D/L variants were characteristic of plants grown under particular illumination levels. The present analysis demonstrated that the scheme including dark adaptation and short readaptation to light (D/L) enabled to obtain generally correct data.