Abstract
Abstract FAB mass spectrometry with MIKES scanning provides an effective means of identifying cyclic nucleotides. Its application readily distinguishes 2′,3′- and 3′,5′-isomers, and has enabled the demonstration of the natural occurrence of ccMP, cUMP, cIMP and cdT'NP in manrmals and higher plants, the identification of novel side-products generated by cytidylyl cyclase activity and of synthetic cyclic nucleotide derivatives, and the quantitation of phosphodiesterase activity.
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