Abstract
Two types of glycerolipids [monoacylglycerols (MAG) and cyclitols] were isolated by reversed phase high-performanceliquid chromatography from the MeOH extracts of a marine sponge, and analyzed by fast atom bombardment mass spectrometry(FAB-MS) in positive-ion mode. FAB mass spectra of these compounds included protonated molecules [M+H]+ and abundantsodiated molecules [M+Na]+ from a mixture of m-NBA and NaI. The structures of these compounds were elucidated by FABcollisional-induced dissociation (CID)-tandem mass spectrometry. To find diagnostic ions for their characterization, these lipidswere analyzed by collision-induced dissociation (CID) B/E-linked scan. The CID B/E-linked scan of [M+H]+ and [M+Na]+ precursorions resulted in the formation of numerous characteristic product ions via a series of dissociative processes. The product ionsformed by charge-remote fragmentation (CRF) provided important information for the characterization of the acyl chain structuresubstituted at the glycerol backbone. Some of product ions were diagnostic for the presence of a glycerol backbone and acylchain structure, respectively.
Highlights
Glycerolipids generally contain saturated fatty acids with even carbon numbers, whereas those extracted from animals are found to possess fatty acids with odd numbered and branched chains.[1]
The product ions formed by charge-remote fragmentation (CRF) provided important information for the identification of the acyl chain structure substituted at the glycerol backbone
FAB spectra of glycerolipids The glycerolipids isolated from the marine sponge are classified by specific types of acyl substituents with differences in hydrocarbon chain length and in the presence and position of unsaturation
Summary
Glycerolipids generally contain saturated fatty acids with even carbon numbers, whereas those extracted from animals are found to possess fatty acids with odd numbered and branched chains.[1]. Abstract: Two types of glycerolipids [monoacylglycerols (MAG) and cyclitols] were isolated by reversed phase high-performance liquid chromatography from the methanol extracts of a marine sponge, and analyzed by fast atom bombardment mass spectrometry (FAB-MS) in positive-ion mode. The product ions formed by charge-remote fragmentation (CRF) provided important information for the identification of the acyl chain structure substituted at the glycerol backbone.
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