Application of encapsulated algae into MBR for high-ammonia nitrogen wastewater treatment and biofouling control

Abstract

Low microbial activity and serious membrane biofouling are still critical problems that hinder the extensive application of membrane bioreactor (MBR) for industrial wastewater treatment. To address these bottlenecks, we report a new specialized microorganism encapsulation strategy for constructing a highly efficient MBR system. In our study, the algae-entrapping fiber macrospheres with polymeric coating were first coupled with membrane separation for treating refractory high-ammonia nitrogen wastewater. In comparison with traditional alginate beads, the developed macrocapsule (~0.5 cm) exhibited higher biomass harvesting and lower microbial leakage because of the confined micro-aerobic environment created by dual encapsulation of rigid inorganic macrosphere and porous polymeric layers. Application of algae-encapsulating macrocapsule to MBR presented excellent chemical oxygen demand (COD) and ammonia nitrogen (NH3-N) removal efficiency of 62.23 and 97.38 %, respectively, which were higher than the corresponding values for algae/SA beads and free algae. The biodegradation performance of NH3-N by encapsulated microalgae was similar or superior to that by free cells when the initial content of ammonia nitrogen ranged from 50 to 100 mg/L. The results well demonstrated that the GFS@polymer macrocapsule as a physical barrier reduced the inhibitory effect of higher concentration ammonia nitrogen on the bioactivity of living cells. Importantly, the encapsulated core-shell macrocapsules showed superior anti-biofouling capacity, which had a membrane resistance of 3-5 times lower than that of cell/alginate beads and free cells. This work will open a new avenue to develop a novel encapsulated MBR for various non-degradable wastewater treatments as an energy-saving and sustainable way.