Abstract
Electroporation provided an effective transformation system for strains of Xanthomonas campestris pv. oryzae. Transformation was observed for strain X37-2 with plasmid pUFR027 over a range of electric field strengths (9.2-17.1 kV/cm) and two different pulse lengths. Optimization of the compensatory relationship between the electrical parameters produced maximal transformation efficiency (3.6×10 9 transformants/μg DNA) and frequency (1.8×10 −2 transformant/survivor) for strain X37-2. When applied to four other strains of X. c. oryaze, these conditions yielded lower levels of transformation (7.8×10 6 to 2.7×10 7 transformants/μg DNA) (...)
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