Application of electrophoresis and western blot to detect IgG in CSF of patients with multiple sclerosis

Abstract

Event Abstract Back to Event Application of electrophoresis and western blot to detect IgG in CSF of patients with multiple sclerosis Asma MEDOURI1, Ines BELLIL1 and Douadi KHELIFI1* 1 Université Constantine 1, Algeria Multiple sclerosis (MS) is a demyelinating autoimmune disease of the central nervous system, characterized by the spread of multiple lesions in the white matter. Its diagnosis is mainly based on clinical and radiological signs, but laboratory tests based on analysis of IgG in cerebrospinal fluid (CSF) and serum are required for diagnosis with more certainty. The purpose of this study is to test the feasibility of adopting the technique of electrophoresis (PAGE: polyacrylamide gel electrophoresis) followed by western blot in the diagnosis of this disease (MS). For this we collected the couples serum / CSF of a population composed of 10 patients aged from 18 to 56 years with clinical and radiological suggestive of MS and controls (n = 3) with other non-inflammatory neurological diseases. The proteins of the two fluids (serum and CSF) are extracted and separated by various variants of PAGE followed by immunoblotting. The comparison between the protein patterns of CSF of patients and those of controls suspects the presence of IgG in the CSF of patients within the zone of high molecular weight on SDS-PAGE (dodecyl sulphate- gel electrophoresis). Using the technique of Western blotting confirmed the presence of IgG in the CSF of patients. An epidemiological study has determined the sex ratio (1: 2.3), the average age (35.68) and the most common form (relapsing-remitting) of this disease. References FROMONT A., BINQUET C, CLERC L et MOREAU T. (2009). Épidémiologie de la sclérose en plaques : la particularité française. 2009. Revue Neurologique. 165 : 671-675. GILLAINA N., FUMAL A ET MINON J-M. (2006). Bandes oligoclonales et index IgG interprété selon Reiber dans les maladies inflammatoires du système nerveux central. Immuno-analyse et biologie spécialisée.21 : 348–356. MERCIER VILLET A., BOREL GIRAUD N, CAUDIE C et RUEL J-H. (2004). Place de l’immunofixation des IgG du LCR dans le diagnostic de sclérose en plaques (SEP).Immuno-analyse et Biologie spécialisée. 19 :89–92 SINGH N.K., SHEPHERED K.W and CORNISH G.B. (1991). A simplified SDS-PAGE procedure for separating LMW subunits of glutenin. Journal of Cereal Science 14:203-208 Keywords: CSF, demyelination, IgG, MS, SDS-PAGE, western blotting Conference: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014), Tlemcen, Algeria, 17 Oct - 20 Oct, 2014. Presentation Type: Poster Presentation Topic: Autoimmunity Citation: MEDOURI A, BELLIL I and KHELIFI D (2014). Application of electrophoresis and western blot to detect IgG in CSF of patients with multiple sclerosis. Front. Immunol. Conference Abstract: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014). doi: 10.3389/conf.fimmu.2014.04.00012 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Sep 2014; Published Online: 01 Dec 2014. * Correspondence: Prof. Douadi KHELIFI, Université Constantine 1, Constantine, Algeria, dkhelifi@yahoo.fr Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Asma MEDOURI Ines BELLIL Douadi KHELIFI Google Asma MEDOURI Ines BELLIL Douadi KHELIFI Google Scholar Asma MEDOURI Ines BELLIL Douadi KHELIFI PubMed Asma MEDOURI Ines BELLIL Douadi KHELIFI Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.