Abstract

Application of effect-surface method in enzymatic hydrolysis of corrugated Paphia undulata by microwave acid was studied. In this experiment, the process was optimized, and the influence of microwave acid combined treatment on enzymatic hydrolysis of corrugated Paphia undulata was explored by means of star-point design and effect-surface analysis. The experimental results show that the acid type and concentration of acid, acid type and power of microwave, acid concentration and microwave power, microwave power and microwave time affect peptide yield, under the optimal process conditions, peptide yield can reach 85.67%, the value and the response surface model prediction is very close to 86.62%, star design - response surface model can be applied to small molecular peptides hydrolyzed well optimization of process conditions.

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