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Abstract
Measurement of water activity and moisture sorption isotherms of foods and biomaterials are important to determine the state of water. In this work, a dynamic temperature-humidity (DTH) controlled chamber was used to measure water sorption isotherm and compared with the conventional isopiestic method. Temperature and relative humidity of DTH chamber can be controlled in the range of -15 to 100°C and 0 to 98%, respectively; thus, measurement of water activity at any point can be measured within the above ranges. The DTH chamber method showed high reproducibility as compared with the conventional isopiestic method when measured isotherms of cellulose, lignin, and hemicellulase were compared at 30°C. Finally, isotherm data of cellulose, lignin, and hemicellulase were generated in the temperature range of 10-90°C using DTH chamber, and these were modelled by BET and GAB equations. The model parameters were correlated with the temperature.
Highlights
Water activity indicates the states of water in foods, and it is a crucial indicator of food stability [1,2,3,4]
Moisture sorption isotherm at a certain temperature indicates the relationship of the water activity and moisture content
In the case of hemicellullase, moisture content remained the same after 20 min (Figure 2(c)), since it was already contained very low moisture. This indicated that the initial moisture content played a role to achieve the required equilibration time to reach initial conditioning
Summary
Water activity indicates the states of water in foods, and it is a crucial indicator of food stability [1,2,3,4]. The conventional static isopiestic method uses desiccator (i.e., an enclosed atmospheric environment of a specific relative humidity) and saturated salt solutions (i.e., creating specific relative humidity environments). It is the most basic and popular method, and it is widely used to measure moisture sorption isotherm of foods. Desiccator was commonly used, and different types of cells, such as glass and metal jars, and plastic containers were used It is a simple method and needs a simple setup (i.e., a desiccator or air tight chamber, different types of salts, and sample holder) [13]. This breaks the equilibrium relative humidity in the desiccator, and it needs time to reach at the set specific
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