Abstract
Research in the field of lower extremity vascular disease has increased in popularity over the years. To adequately characterize and validate the effectiveness of vascular interventions, in vivo experimentation in large animals is required. Thus, it is necessary to find a method to detect the shape and density of blood vessels in the lower extremities that can evaluate and verify the treatment measures' effectiveness and have high accuracy and repeatability. This study characterized factors that determined both the accuracy and overall value of digital subtraction angiography in lower limb arteriography using a canine animal model. Six beagle dogs were anesthetized and immobilized on the motorized table. The femoral artery was accessed using an indwelling needle. A bolus of contrast agent was injected into the access site, and digital subtraction angiography with bolus chase technology was used to collect contrast images for analysis. At the end of the procedure, the anesthetized dogs were euthanized using an overdose of potassium chloride. After confirming the euthanasia of the dogs, the cadavers were taken to the experimental animal center of Xinjiang Medical University and processed by qualified institutional personnel. The final arteriographic images of the hind limbs from all six dogs were precise, and the branches of small vessels could be distinguished without any visible artifacts. These results suggested that arteriography using digital subtraction angiography could reveal the shape and density of blood vessels in canine animal models. This method has great potential to significantly improve research related to limb ischemia due to its simple and reproducible results.
Highlights
Research in the field of lower extremity vascular disease has increased in popularity over the years
RESULT: The final arteriographic images of the hind limbs from all six canines were clear, and the branches of small vessels could be distinguished without any visible artifacts. These results suggest that arteriography using digital subtraction angiography can clearly reveal the shape and density of blood vessels in canine animal models
This method has great potential to significantly improve research related to limb ischemia, due to its simple and reproducible results
Summary
Research in the field of lower extremity vascular disease has increased in popularity over the years. It is necessary to find a method to detect the shape and density of blood vessels in the lower extremities, which can evaluate and verify the effectiveness of the treatment measures, and has high feasibility and repeatability This experiment is to characterize the influencing factors and determine both the feasibility and overall value of digital subtraction angiography in lower limb arteriography in a canine animal model. Animal models simulating acute and chronic ischemia of the lower limbs are established through ligation, cutting and embolization of the arteries These methods are becoming of great interest to the research community, both locally and internationally, in order to explore the best diagnosis and treatment approaches for peripheral arterial diseases[3].Due to operational feasibility and experimental costs, the most widely used animal models are rabbits and rats, despite the size of their blood vessels and skeletons being significantly different from that of humans. There are many limitations in simulation studies, especially in the current study of Ilizarov bone transfer technology for the treatment of peripheral artery disease.These simulation studies, provide an ideal platform to observe and carry out research in this field
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