Abstract
This work focuses on a quantitative analysis of sucrose using diffusion ordered-quantitative 1H-nuclear magnetic resonance spectroscopy (DOSY-qNMR), where an analyte can be isolated from interference based on its characteristic diffusion coefficient (D) in gradient magnetic fields. The D value of sucrose in deuterium oxide at 30°C was 4.9×10−10m2/s at field gradient pulse from 5.0×10−2 to 3.0×10−1T/m, separated from other carbohydrates (glucose and fructose). Good linearity (r2=0.9999) was obtained between sucrose (0.5–20.0g/L) and the resonance area of target glucopyranosyl-α-C1 proton normalised to that of cellobiose C1 proton (100.0g/L, as an internal standard) in 1D sliced DOSY spectrum. The DOSY-qNMR method was successfully applied to quantify sucrose in orange juice (36.1±0.5g/L), pineapple juice (53.5±1.1g/L) and a sports drink (24.7±0.6g/L), in good agreement with the results obtained by an F-kit method.
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