Abstract

Abstract Discerning the geometry of spheroidal scatterers of micron order is an important topic in identifying marine microbes. Optical diffraction tomography theory indicates that under the first-order Born approximation for weak scattering, scattering amplitude in the far zone and scattering potential of the scatterer have a Fourier relationship. In this paper, we describe a method based on diffraction tomography theory and determine the size and the shape of spheroidal scatterers by reconstructing the distribution of scattering potential from angular resolved scattered field. As a demonstration of this method, the scattering from spheroidal particles with equal-volume-sphere radii of 0.5429, 1.00, and 2.00 μm and an aspect ratio that varies from 0.4 to 1.5 was modeled by using T-matrix theory and used as test data. Simulation results show that in the case of low contrast, size and shape determination can be achieved with sub-wavelength precision.

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