Application of Differential Electrodes in a Dielectrophoresis-Based Device for Cell Separation

Abstract

In this paper, a microfluidic device is introduced based on direct current (dc) dielectrophoresis for the separation of circulating tumor cells from normal blood cells. Differential sidewall electrodes are designed inside the channel and two middle-channel outlets are located after the end of the electrodes, which are assigned to collect the normal blood cells. A maximum dc potential of 3 V is applied to the electrodes, resulting in a differential dielectrophoretic forces acting on the cells. This paper involves isolating MCF-7 breast cancer cells from white blood cells (WBCs) and red blood cells (RBCs), eliminating the demand for primary RBC lysis. Numerical simulation results revealed that the differential electrodes not only suppressed successfully the need for high applied voltages by dc dielectrophoresis but also diminished the joule heating effects. Besides that, the arrangement of the differential electrodes was observed to play a critical role in the separation performance. In particular, designing the outlets, as well as adjusting the electrode potentials, has effectively assisted the device to be tunable for different cancer cells even with different radii. More strikingly, the ability of the device to deflect the waste cells (WBCs and RBCs) to out of the microchannel prior to the cancer cells was proven to positively affect the separation efficiency and purity. The proposed device was capable of separating different cells leveraging various diameters with capture purity and efficiency of higher than 83% and 100%, respectively.