Abstract
Telomerase plays an important role in abnormal cell proliferation, metastasis, stem cell maintenance, and immortality in different types of cancer. As a result, the design of drugs targeting telomerase is of great significance for the treatment of cancer. In recent years, treatment strategies for telomerase have attracted extensive attention. However, finding an effective treatment is still more difficult. Understanding the structure, function and biological process of telomerase and their related proteins is of great help to drug development and clinical treatment. As a result, this research mainly introduces different biological detection methods of telomerase, including PCR, western blot, CCK-8 assay, sphere formation assay, immunocytochemistry and nanobiotic detection technology. By understanding the detection range, different aspects of detection, and advantages and disadvantages of several detection methods, the development of telomerase detection technology can be well understood. And it can better understand the activity and expression of telomerase, and hope to provide reference for future drug development targeting telomerase, telomerase and related proteins.
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