Application of DEPArrayTM technology for the isolation of white blood cells from cell mixtures in chimerism analysis

Abstract

After allogeneic bone marrow or blood stem cell transplantation, STR profiling is often used for monitoring chimerisms. For the evaluation reference samples taken from the patient before the transplantation and/or taken from the donor are needed. In the case described here, no reference samples were available. Reporting the chimerism based on posttransplantation samples did not yield conclusive results;therefore, white blood cells and epithelial cells obtained from a buccal swab taken from the patient were separated using DEPArray(TM) technology and STR profiling was carried out resulting in distinct reference profiles.