Abstract
• Inhibition of wild-type DNA release from leukocytes for liquid biopsy. • γ-Cyclodextrin (γ-CyD)/polyethylene glycol (PEG) is a potential additive. • γ-CyD/PEG suppress the wild-type DNA release from leukocytes. • γ-CyD/PEG is applicable for blood collection tubes for liquid biopsy. In recent years, liquid biopsy has garnered considerable attention as a valuable diagnostic technology for cancer. An analysis target of liquid biopsy is cell-free DNA; however, contamination with wild-type DNA released from injured leukocytes leads to low test accuracy. Thus, there is a need for blood-storage tools that inhibit the release of wild-type DNA from leukocytes. In the present study, we demonstrated that γ-cyclodextrin (γ-CyD) and polyethylene glycol (PEG) synergistically inhibit DNA release from cells by inhibiting cell aggregation. In addition, formation of a network structure of PEG via the inclusion complexation of γ-CyD with two PEG chains is probably important for the inhibitory effects on DNA release. These findings indicate that the combination of γ-CyD and PEG has potential as a blood-storage tool for liquid biopsy.
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