Application of CRISPR/Cas9 Technology in the Genetic Improvement of Rice Yield and Quality Characters

Bo Peng,Mengyang Zheng,Xiaohua Song,Quanxiu Wang,Hongyu Yuan,Xiayu Tian,Wei Zhou,Juan Peng,Tao Yang,Jintao Li,Xinxiang A,Yanfang Sun,Ruihua Pang,Xiaorui Ma

doi:10.5296/jbls.v11i2.17039

Abstract

Rice (Oryza sativa L.) is one of the most important food crops in the world. With the continuous increase of global population and the continuous improvement of people's living standards, it has become the focus of attention of rice breeders and botanists to improve the yield and quality of rice. In the new genome editing technology, the CRISPR/Cas system can accurately and specifically edit the target genome region. In recent years, CRISPR/Cas9 technology has made important progress in studying the function of new genes, increasing rice yield and improving rice quality. Therefore, this paper mainly reviewed the application of CRISPR/Cas9 technology in the genetic improvement of rice yield and rice quality in recent years, and looked forward to the application prospect of CRISPR/Cas9 technology in rice genetic breeding, which will provide important reference for the cultivation of new high-quality and high-yield rice varieties.

Highlights

Rice (Oryza sativa L.) is one of the most important food crops in the world
This paper mainly reviewed the application of CRISPR/Cas9 technology in the genetic improvement of rice yield and rice quality in recent years, and looked forward to the application prospect of CRISPR/Cas9 technology in rice genetic breeding, which will provide important reference for the cultivation of new high-quality and high-yield rice varieties
The results showed that the plant height and ear length of dep1 mutant decreased by 20% compared with the control, but the number of flowers per ear increased by 50% compared with the control

Summary

Introduction

Rice (Oryza sativa L.) is one of the most important food crops in the world. It is the main food for more than 3 billion people in more than 100 countries and regions (Kusano et al, 2015; Peng et al, 2016a). CRISPR/Cas9 gene editing technology has developed rapidly in just a few years due to its advantages of simple operation, high editing efficiency, support for multi-target editing and various editing forms, and has been widely used in a variety of organisms, making an important contribution to the study of gene function and the inheritance and improvement of important traits of animals and plants.

Results

Conclusion