Application of combined detection of serum tumor markers, Ki-67 and P-glycoprotein in lymph node metastasis and postoperative recurrence of colorectal cancer patients

Abstract

Objective To analyze the effect of combined detection of serum tumor markers, Ki-67 and P-glycoprotein (PGP) in lymph node metastasis and postoperative recurrence of colorectal cancer patients. Methods The clinical data of 517 colorectal cancer patients who had underwent surgical treatment were retrospectively analyzed. Among them, lymph node metastasis was found in 165 cases (lymph metastasis group), and 352 cases did not have lymph node metastasis (non lymphoid metastasis); postoperative recurrence was found in 224 cases (postoperative recurrence group), and 293 cases did have postoperative recurrence (non postoperative recurrence group). The serum tumor markers levels of cancer antigen 19-9 (CA19-9), carcinoembryonic antigen (CEA), cancer antigen 125 (CA125) and the expressions of Ki-67, PGP were compared. The correlation between the serum tumor markers levels and the expressions of Ki-67, PGP was analyzed. Results The serum levels of CEA, CA19-9 and CA125 and the positive expression rates of Ki-67 and PGP in lymph metastasis group were significantly higher than those in non lymph metastasis group: (21.39 ± 3.15) μg/L vs. (10.12 ± 2.48) μg/L, (68.48 ± 5.82) U/L vs. (35.26 ± 3.51) U/L, (82.16 ± 7.53) U/L vs. (32.46 ± 6.24) U/L, 69.70% (115/165) vs. 13.64% (48/352) and 72.73% (120/165) vs. 14.77% (52/352), and there were statistical differences (P<0.01). The serum levels of CEA, CA19-9 and CA125 and the positive expression rates of Ki-67 and PGP in postoperative recurrence group were significantly higher than those in non postoperative recurrence group: (18.26 ± 2.34) μg/L vs. (9.18 ± 1.26) μg/L, (47.52 ± 4.85) U/L vs. (21.43 ± 2.18) U/L, (59.16 ± 4.25) U/L vs. (33.17 ± 3.46) U/L, 60.27% (135/224) vs. 9.56% (28/293) and 6.70% (15/224) vs. 7.51% (22/293), and there were statistical differences (P<0.01). The correlation analysis results showed that the positive expression rates of Ki-67 and PGP were positive correlated with the serum levels of CEA, CA19-9 and CA125 (P < 0.05). Conclusions The levels of serum tumor markers and the positive expression rates of PGP and Ki-67 in colorectal cancer patients with lymph metastasis and postoperative recurrence are high. The levels of tumor markers are closely related to the positive expression rates of Ki-67 and PGP, which indicates that the combined detection of these indexes has a good effect on evaluating lymph metastasis and postoperative recurrence of colorectal cancer patients. Key words: Colorectal neoplasms; Neoplasm metastasis; Tumor markers, biological; P-glycoprotein; Ki-67