Application of chitosan-gelatin-carbonate hydroxy apatite scaffold toward the number of osteoblasts in alveolar bone defects in Wistar Rats

Abstract

Tooth extraction may cause bone defects that require regenerative therapy. Chitosan, gelatin, and carbonate hydroxyapatite can help increase osteoblast proliferation and differentiation, which plays a role in the bone regeneration process. Scaffold made from chitosan-gelatin carbonate hydroxyapatite has promising characteristics that may help enhance the bone regeneration process. This study aimed to determine the effect of chitosan-gelatin carbonate hydroxyapatite (CG:CHA) scaffold application towards the number of osteoblasts on alveolar bone defect in Wistar rats after tooth extraction. In vivo experimental laboratory research conducted with post-test-only control group design. The Wistar rats were divided into 6 groups, tooth extraction was conducted, and in the treatment group the C-G:CHA scaffold was applied on the sockets afterward. After 7, 14, and 21 days of scaffold application, jaw resection was done to observe the number of osteoblasts by HE histological examination. The result data were analyzed using a one-way ANOVA test. Data analysis using one-way ANOVA test results in P=0 (P <0,05) which indicates a significant difference between the control and treatment groups. The chitosan-gelatin carbonate hydroxyapatite (C-G:CHA) scaffold can increase the number of osteoblasts.