Abstract
<p>Abstract<br />This research were to obtain the optimal concentration of chitosan as an antibacterial material wich had the longest of storage period of pangasius fillet at low temperature storage (5-10oC). The method used is an experimental method with a completely randomized design, four treatments and three replications. Pangasius fillet was soaked with chitosan 0%, 1%, 2% and 3% for 3 minutes, then packed with styrofoam and plastic wrap and stored at low teperature. The observation were made on day 1st, 3rd, 5th, 6th ,7th, 9th,10th, 11th and 12th. The parameters observed total bacterial colonies, the degree of acidity (pH), weight loss, and antibacterial activity of chitosan using disc diffusion methods. The conclusion of research was that the 2% chitosan is optimal concentration for pangasius fillet storage at a low temperature until day 11th, with a total 6.7 X105 cfu/g of bacterial colonies, pH 6.67 and total 6,78 % of weight loss.Chitosan also has a higher antibacterial capability against Escherichia coli (Gram negative bacteria) than Staphylococcus aureus and Bacillus subtilis (Gram positive bacteria).</p>
Highlights
This research were to obtain the optimal concentration of chitosan as an antibacterial material wich had the longest of storage period of pangasius fillet at low temperature storage (5-10oC)
The conclusion of research was that the 2% chitosan is optimal concentration for pangasius fillet storage at a low temperature until day 11th, with a total 6.7 X105 cfu/g of bacterial colonies, pH 6.67 and total 6,78 % of weight loss.Chitosan has a higher antibacterial capability against Escherichia coli (Gram negative bacteria) than Staphylococcus aureus and Bacillus subtilis (Gram positive bacteria)
Fakultas Perikanan dan Ilmu Kelautan Universitas Padjadjaran
Summary
Bahan yang digunakan pada penelitian ini adalah akuades steril, alkohol 95%, antibiotik tetrasiklin, asam asetat 1 m, BaCl2 (Merck) 1%, buffer ph 4 dan 7, kertas cakram, es curai, H2SO4 (Merck) 1%, ikan patin yang berukuran 350-400g/ekor sebanyak 16 ekor, serbuk kitosan, NaCl fisiologis, nutrien agar, nutrient broth, kultur murni bakteri Eschericia coli, Staphylococcus aureus, dan Bacillus subtilis. Alat yang digunakan pada penelitian ini adalah plastik yang berisi air dan oksigen untuk mengangkut ikan patin dari BLUPPB Karawang, bak fiberglass dan aerator, cawan petri, inkubator, gelas ukur, magnetic stirrer, pipet, oven, timbangan digital dengan ketelitian 0,1 g, plastic wrap, ph meter, dan refrigerator
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