Abstract
Cell-free protein synthesis (CFPS) has become an established tool for rapid protein synthesis in order to accelerate the discovery of new enzymes and the development of proteins with improved characteristics. Over the past years, progress in CFPS system preparation has been made towards simplification, and many applications have been developed with regard to tailor-made solutions for specific purposes. In this review, various preparation methods of CFPS systems are compared and the significance of individual supplements is assessed. The recent applications of CFPS are summarized and the potential for biocatalyst development discussed. One of the central features is the high-throughput synthesis of protein variants, which enables sophisticated approaches for rapid prototyping of enzymes. These applications demonstrate the contribution of CFPS to enhance enzyme functionalities and the complementation to in vivo protein synthesis. However, there are different issues to be addressed, such as the low predictability of CFPS performance and transferability to in vivo protein synthesis. Nevertheless, the usage of CFPS for high-throughput enzyme screening has been proven to be an efficient method to discover novel biocatalysts and improved enzyme variants.
Highlights
Biocatalysis and biocatalytic processes affect many industries ranging from consumer products and food to the chemical and pharmaceutical industry
Cell-free protein synthesis (CFPS) systems might contribute to accelerating the development process of new enzymes and enable rapid screening for suitable biocatalysts
We have shown that CFPS bears a high value for biocatalytic processes and has become an accepted alternative for in vivo expression systems
Summary
Biocatalysis and biocatalytic processes affect many industries ranging from consumer products and food to the chemical and pharmaceutical industry. Cell-free protein synthesis (CFPS) systems might contribute to accelerating the development process of new enzymes and enable rapid screening for suitable biocatalysts. Lima-Ramos et al, economic analysis should be carried out at an early development phase of the Catalysts 2019, 9, 190; doi:10.3390/catal9020190 www.mdpi.com/journal/catalysts. This means that means the choice theand biocatalyst improvement in order to in tune the production [13]. To to tune the biocatalyst’s to requirements the process requirements required at an modify proteins facilitates the facilitates development and implementation of biocatalyzed syntheses in. The availability genes and genomes originating from metagenomes natural poolspools or mutational creation is steadily increasing [17].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
