Application of carboxymethyl-β-cyclodextrin as a chiral selector in capillary electrophoresis for enantiomer separation of selected neurotransmitters

Abstract

The aim of this work was to optimize conditions for capillary electrophoresis separation of different neurotransmitters (serotonin, phenylalanine, dopamine, adrenaline, ephedrine, propranolol and DOPA) in a single run, including separation of existing enantiomers. As chiral selectors added to the borate background, electrolyte unsubstituted α-, β- and γ-cyclodextrins (CDs), methyl-, dimethyl-, and trimethyl-substituted β-CDs, and hydroxypropyl-substituted α-, β- and γ-CDs were examined. Also carboxymethyl-β-CD and succinyl-β-CD were used for this purpose. In addition to the kind and concentration of chiral selector, some other experimental factors also have been optimized, such as concentration of borate buffer, content of methanol, pH of electrolyte, method of sample introduction into the capillary and washing procedure between consecutive runs. The best results were obtained using 20 m M carboxymethyl-β-CD in borate buffer of pH 7.5 as running electrolyte and hydrostatic injection. The obtained sensitivity of response (peak height) varied from 0.4 for adrenalines to 2.3 mAU m M −1 for propranolols. The concentration detection limits ( S/N=3) were in the range from 0.04 m M for propranolols to 0.2 m M for adrenalines. The resolution obtained in optimized conditions in a single run was from 0.75 for adrenalins and 1.0 for propranolols up to 2.0 for ephedrines. The developed method was employed for determination of these analytes in brain tissue extracts.