Abstract
Capillary electrophoresis using laser-induced fluorescence detection (CE-LIF) is one of the most sensitive separation tools among electrical separation methods. The use of CE-LIF in immunoassays and enzyme assays has gained a reputation in recent years for its high detection sensitivity, short analysis time, and accurate quantification. Immunoassays are bioassay platforms that rely on binding reactions between an antigen (analyte) and a specific antibody. Enzyme assays measure enzymatic activity through quantitative analysis of substrates and products by the reaction of enzymes in purified enzyme or cell systems. These two category analyses play an important role in the context of biopharmaceutical analysis, clinical therapy, drug discovery, and diagnosis analysis. This review discusses the expanding portfolio of immune and enzyme assays using CE-LIF and focuses on the advantages and disadvantages of these methods over the ten years of existing technology since 2008.
Highlights
Capillary electrophoresis (CE) has become an important tool in the era of separation since its first introduction by Jorgenson and Lukacs in 1981 [1]
Bioassays using native fluorescent proteins and peptides will be excluded from this review, as we focus on the discussion of immunoassays and enzyme assays, as they both rely on the use of fluorescent antigens or antibodies and substrates
Our method enabled the quantification of the concentration of anti-cyclic citrullinated peptides (CCP) antibodies in patient sera, ranging from 0.1–0.4 μg/mL (Figure 3)
Summary
Capillary electrophoresis (CE) has become an important tool in the era of separation since its first introduction by Jorgenson and Lukacs in 1981 [1]. CE-LIF performances are generally fast, automated, require a small number of samples, and are highly sensitive, they enable the simultaneous separation of various compounds at different sizes under minute records. The use of CE-LIF in bioanalytical assays has drawn significant attention with the publication of numerous papers dealing with the analysis of biomolecules based on the two significant bio-reaction classes:. We discuss the application of CE-LIF technique in the analysis of proteins and peptides, with an emphasis on immunoassays and enzyme assays in the last decade, from 2008 to early 2019. The details of the instrument conditions, method developments, and advances in the CE-LIF-based assay platforms in the biological studies are reviewed
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