Abstract
To investigate the applicability of bubble column fermentors to the submerged culture of mushroom mycelia, cultivation of Schizophyllum commune IFO-4928 for production of l-malic acid was carried out using a standard bubble column and an external-loop airlift column. Maximum yields of l-malic acid were attained at a superficial air velocity of 0.7 cm/s for the standard bubble column and 1.7 cm/s for the external-loop airlift column. The optimum temperature was 27°C for both types of column, and the optimum concentrations of both calcium carbonate and glucose were 50 g/ l. The yield of l-malic acid under opimum conditions for the airlift column was larger than for the standard bubble column. From the comparison of the production rates of l-malic acid in flask culture, a jar fermentor, an airlift column, and a standard bubble column, the airlift bubble column was found to be most suitable for the culture of S. commune for l-malic acid production.
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