Application of BS4-enzyme on the methane production from mixture of cattle manures and waste paper

Tuti Haryati,B Listian,A.p Sinurat ,H Hamid ,T Purwadaria

doi:10.14334/jitv.v21i4.1524

Abstract

Cellulose from abundant newspaper waste could be transformed into methane through anaerobic fermentation. This research was carried out to compare the gas production including methane between samples containing feces and waste paper mixture as inoculum and substrate, respectively and added with and without BS4 enzyme. The enzyme was produced in Indonesian Research Institute of Animal Produce (IRIAP) by growing Eupenicillium javanicum BS4 in coconut meals. There were three treatments, i.e., 30% manure (M30); 15 % manure + 15 % paper waste (MP 30); MP30 + 3 mL BS4 enzyme equal to 0.42 U/g dry matter (MPE30) The percentage of waste papers addition in feces was calculated on dry matter (DM) basis and every treatment had five replications. Total gas and methane productions were measured weekly, while dry matter losses were determined during 5 week fermentation. Interactions between treatments and incubation time were analyzed using completely randomized design each week. Kind of substrates influenced both total gas and methane productions during incubation time. Both waste papers and enzyme addition enhanced gas production. The highest total gas and methane productions for five weeks incubation were highly significantly observed (P&lt;0.01) in MP30 and MPE30 compared to M30. Addition of enzyme significantly increased total gas and methane productions in the first week. The highest methane and total gas yield/g dry matter were obtained by BS4 enzyme addition. It was concluded that BS4 enzyme is good in accelerating and increasing the transformation efficiency of waste paper and manure mixture for biogas production.Key Words: Methane, Fibrenolytic-Enzyme, Waste Papers, Cattle Manures

Highlights

Waste paper, including newspapers, is one of the types of waste that are most commonly found in the environment
Cattle manure is usually used as a source of inoculum in the anaerobic production of methane biogas
The research reported in this paper explored the possibility to optimize methane production from waste papers and cattle manure as the substrates and inoculum respectively with the addition of enzyme

Summary

INTRODUCTION

Waste paper, including newspapers, is one of the types of waste that are most commonly found in the environment. Cattle manure is usually used as a source of inoculum in the anaerobic production of methane biogas. The cellulolytic and hemicellulolytic bacteria in the cattle manure transform the cellulose and hemicellulose into monosacharides such as glucose which was transformed by other bacteria into simple organic acids. These organic acids are processed into methane by methanogenic microbes (Ofoefule et al 2010). The research reported in this paper explored the possibility to optimize methane production from waste papers and cattle manure as the substrates and inoculum respectively with the addition of enzyme

MATERIALS AND METHODS

Methane

CONCLUSION