Abstract
We have designed an amperometric xanthine minibiosensor based on a supported biotinylated phospholipid membrane. To this membrane streptavidin-modified xanthine oxidase was coupled. The fabricated biosensor corresponds in shape and size to a needle of 0·3 mm in diameter. The assay is based on the electrochemical detection of enzymatically generated hydrogen peroxide. The response to xanthine was linear up to 1 mmol.l −1 with a detection limit of 0·02 mmol.l −1. The response time was less than one minute and the biosensor was stable for at least five days.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
