Abstract
Abstract Aptamer with small molecular weight, simple structure, and easy synthesis, which can be used repeatedly and preserved for long time, has important applications in biosensor field. This article describes several commonly used methods for fixing aptamer onto the sensor surfaces, including the gold-sulfur self-assembled monolayer, covalent bond, biotin/avidin affinity, complementary nucleic acid chain connection, as well as current research progress and the characteristics of each method. Development of aptasensors with wide detection range, low detection limit, short detection time, and capability of detection of multitargets will certainly facilitate its application in food quality and safety testing, disease diagnosis, drug analysis, environment monitoring, etc.
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