Application of biodegradable seedling trays in paddy fields: Impacts on the microbial community

Abstract

Biodegradable plastics have been widely introduced into agricultural production, but their impacts on the soil ecosystem remain unclear. The present study investigated the impacts of a biodegradable seedling tray (BST) on the microbial communities in paddy soils. A 110-day rice culture experiment was conducted with three different paddy soils developed from black soil (BS, black chernozem soil), chao soil (CS, Fluvo-aquic) and red soil (RS, Alfisols) and three application rates of BST (0, 0.02 and 0.2 g kg−1). Soil phthalic acid ester (PAE) concentrations, physicochemical properties and enzyme activities were determined to evaluate the influence of BSTs on soil quality. 16S high-throughput sequencing was used to study bacterial community composition and the Biolog EcoPlate™ test was used to profile microbial activity and community function. Results show that the application of BSTs did not markedly affect soil quality, and the potential release of PAEs from BSTs was negligible. Interestingly, the microbial community was affected by BSTs in a soil-dependent and time-dependent pattern. The microbial community in RS was not significantly influenced by BSTs. Relative abundances of some predominant genera in BS (e.g. norank_f__BSV40) and CS (e.g. Norank_f__Nitrosomonadaceae) were significantly influenced by BSTs, and db-RDA results show that community composition in BS and CS was shaped mainly by BSTs. Community level profiling shows that BSTs significantly increased microbial activity and decreased functional diversity in BS after 55 days, but the impacts disappeared after 110 days. The results contribute to the knowledge of how biodegradable plastics influence microbial communities in paddy fields and provide information relevant to the practical use of BSTs under field condition.