Abstract

Anthracnose is a disease that attacks papaya fruit while in the field and continues until the fruit is harvested and distributed to the market. Biofungiside made from citronella and green betel was expected to extend the selling papaya at inhibiting the growth of anthracnose. The study was aimed to determine the appropriate concentration of extracts of citronella leaves (Cymbopogon nardus L.) and green betel leaves (Piper betle L.) in inhibiting the growth of C. gloeosporioides as a cause of anthracnose. The extraction was done by boiling the leaves. The test methods used were in vitro and in vivo tests. In vitro tests were carried out to test the effectiveness of citronella leaf extract with concentrations of 5%, 10%, and 10%, 20% concentrations of green betel leaf extract in inhibiting the growth of C. gloeosporioides. The results of the in vitro test showed a significant difference (á = 0.5%) between treatments with the highest effectiveness value being 10% citronella extract with 47.22% growth inhibition of C. gloeosporioides and an average colony diameter of 3.150 cm after 15 days of incubation. The results of the in vivo test, showed significantly different results (á = 0.5%) between papaya fruit soaked with 10% citronella extract and controls, both in the incidence and spread of the disease. The spread of disease in treated papaya fruit was 13.72% while control papaya fruit reached 68.49% at 6 days of storage. Citronella extract with a concentration of 10% can extend the selling time of Calinna papaya (80% maturity index) 6 days longer than the control.

Highlights

  • Antranoksa merupakan penyakit buah pepaya yang menyerang saat di lahan dan berlanjut sampai dipanen yang membuat masa jualnya menjadi singkat

  • Biofungiside made from citronella

  • The study was aimed to determine the appropriate concentration of extracts of citronella leaves

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Summary

Tahap Persiapan

IPB 9) didapatkan dari kebun Sabisa Farm di wilayah kelurahan Loji, kecamatan Bogor Barat, kota Bogor dengan kematangan 80%, panjang 2528 sentimeter, diameter 27-32 centimeter, serta massa rata-rata adalah 800 -1000 gram. Daun serai wangi (Cymbopogon nardus L.) begitu juga daun sirih hijau (Piper betle L.) basah didapat dari kebun Unit Konservasi juga dari Budidaya Biofarmaka (UKBB) kebun Cikabayan Kampus IPB Dramaga. Isolat C. gloeosporioides diperoleh dari Klinik Tanaman Departemen Proteksi Tanaman IPB Dramaga. Natrium hipoklorit 1%, spritus, serta media Potato Dextrose Agar (PDA)

Prosedur Penelitian
Pengujian secara in vitro
Uji in vivo daya hambat ekstrak biofungisida
Pengamatan penyakit antraknosa
Pengukuran warna kulit buah
Pertumbuhan koloni
Pengujian in Vivo
Kejadian penyakit
Jingga dan putih
Penyebaran penyakit pada permukaan buah
Perubahan warna kulit buah
Susut Bobot
Findings
DAFTAR PUSTAKA
Full Text
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