Application of bacteriophages isolated from sewage to control urinary O157:H7 Escherichia coli and several bacterial uropathogens

Salwa Mahmoud Masoud ,Hend A Refat ,Ahmed A Dosocky ,Dina M Mekawy ,Katren T Welliam ,Rehab Mahmoud Abd El-Baky ,Nada S Sayed ,Mohamed A H Abdel Wahab ,Mohamed K Abd-El Aal ,Zeinab S Mohammed ,Basma A Atya ,Zeinab Shawky Hashem

doi:10.21608/nrmj.2020.118451

Salwa Mahmoud Masoud , Hend A Refat + Show 10 more

Open Access

https://doi.org/10.21608/nrmj.2020.118451

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Abstract

Urinary tract infections (UTI) are global bacterial infections. Since the spread of antibiotic resistance, it is necessary to find alternative antimicrobial agents. The aim of this work was to investigate the effect of waste water bacteriophages on the growth of some multi-drug resistance Escherichia coli and other bacterial uropathogens. Urine samples were collected from 30 UTI patients at Minia University Hospital, Minia, Egypt. Twenty Escherichia coli strains were isolated from UTI patients. The Kirby-Bauer disc diffusion method was used to determine the isolates antibiotic susceptibility. The isolates showed resistance to ciprofloxacin and levofloxacin by 70%. Five E. coli bacteriophages were isolated from sewage water samples, tested for their host range and then examined by Transmission electron microscopy (TEM). The TEM examination revealed T4 like bacteriophages. The bacteriophages demonstrated lytic activities against the tested multidrug resistant clinical uropathogenic O157:H7 and non-O157 E. coli isolates, E. coli O157:H7 ATCC 43894, E. coli NRRL B-3008 and, Pseudomonas aeruginosa ATCC 27853 strain, but showed no activity against Klebsiella pneumoniae ATCC10031 and Staphylococcus aureus ATCC 6538. This study revealed that bacteriophages could act as effective alternatives of antibiotics especially against multidrug resistant bacteria; however, further in-vivo and shelf stability studies are needed.

Highlights

Urinary tract infections (UTIs) are one of the most prevalent bacterial infections either in the community or hospital acquired (Hryniewicz et al, 2001)
2020 revealed that UTI is the infection of the kidneys, ureters, bladder and/ or urethra by bacterial invasion of the urinary tract that leads to an inflammatory response of the urothelium
Standard strains used in this study were E. coli O157:H7, E. coli NRRL B-3008, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 10031 and Staphylococcus aureus ATCC 6538

Summary

Introduction

Urinary tract infections (UTIs) are one of the most prevalent bacterial infections either in the community or hospital acquired (Hryniewicz et al, 2001). Several studies of Ramesh et al, (2008); Beyene and Tsegaye, (2011) reported that E. coli has been and is still the most frequent etiological agent of UTIs, which accounts for more than 80% of community acquired, 50% of nosocomial and more than 80% of cases of uncomplicated pyelonephritis. Phages that can infect more than one species are known as polyvalent host range bacteriophages, as demonstrated by Carvalho et al, (2010); Gill and Hyman, (2010). Leibovici et al, (1998) documented that polyvalent and broad host range phages have the advantage of acting as broad spectrum antibiotics, which can infect many bacteria without the need for testing the sensitivity of bacteria to these bacteriophages

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