Abstract
BackgroundElectrospinning is an easy and effective technique to produce submicron fibers possessing a range of attractive characteristics such as interconnected porous structures similar to natural ECM and good resilience to movement. Rapid and efficient cell attachment to nanofibrous matrices is a necessary prerequisite in tissue engineering. Thus, the aim of this study is to evaluate poly(ε-caprolactone-co-lactide)/Pluronic (PLCL/Pluronic) nanofibrous matrices with avidin-biotin technology for improving cell adhesion for the first time.ResultsPLCL/Pluronic nanofibers had relatively homogeneous fibers and interconnected porous structures. Pluronic significantly modified the hydrophilicity of nanofibrous matrices and PLCL/Pluronic nanofibrous matrices had better performance on maintaining cell proliferation. Avidin-biotin technology had no negative effect on the hydrophilic property, mechanical property and cell proliferation. Meanwhile, the attachment and spreading of adipose-derived stem cells (ADSCs) onto PLCL/Pluronic nanofibrous matrices with avidin-biotin technology was promoted obviously.ConclusionsPLCL/Pluronic nanofibrous matrices inheriting the excellent characteristics of both PLCL and Pluronic have the better cell adhesion ability through avidin-biotin technology, implying a promising application in skin care, tissue regeneration and other related area.
Highlights
Electrospinning is an easy and effective technique to produce submicron fibers possessing a range of attractive characteristics such as interconnected porous structures similar to natural extracellular matrix (ECM) and good resilience to movement
The aim of the current study was to explore the possibility of using the avidin-biotin binding system (ABBS) and fabricating avidin-biotin-PLCL/Pluronic nanofibrous matrices for skin care application
Electrospun scaffolds with microscale porous structures are most favorable for tissue engineering scaffolds because they are a network of interconnected pores that provides nutrients and gas exchange and cellular infiltration, which are crucial for cell viability and tissue regeneration
Summary
Electrospinning is an easy and effective technique to produce submicron fibers possessing a range of attractive characteristics such as interconnected porous structures similar to natural ECM and good resilience to movement. Rapid and efficient cell attachment to nanofibrous matrices is a necessary prerequisite in tissue engineering. The common treatments for skin defect repair are allogeneic skin graft, mesh skin graft and split-thickness skin graft [1, 2]. These approaches have several shortcomings, including graft contraction, secondary donor site injury, immune rejection and graft dysfunction. The basic strategy of tissue engineering is to fabricate a scaffold to mimic the natural extracellular matrix (ECM) [3]. The skin extracellular matrix consists of micron to submicron fibrils network of structural and regulatory proteins produced by encapsulated cells to form matrix
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