Abstract
The atomic force microscope (AFM) is a state-of-the-art tool that can analyze and characterize samples on a scale from angstroms to 100 μm by physical interaction between AFM cantilever tip and sample surface. AFM imaging has been used incrementally over last decade in living cells in cardiovascular research. Beyond its high resolution 3D imaging, AFM allows the quantitative assessments on the structure and function of the underlying cytoskeleton and cell organelles, binding probability, adhesion forces, and micromechanical properties of the cell by "sensing" the cell surface with mechanical sharp cantilever tip. AFM measurements have enhanced our understanding of cell mechanics in normal physiological and pathological states.
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