Abstract
In this study, the AMTPSs which composed of different types and concentrations of a single nonionic surfactant (i.e. Pluronic L-121, L-81, L-61, and L31) were formed and their respective cloud point temperature was determined. The clouding phenomenon was highly dependent on the molecular weight of the hydrophobic polypropylene oxide of the Pluronic surfactants. The extraction of bioactive compounds from the Carica papaya leaves using the temperature-induced aqueous micellar two-phase system (AMTPS) was demonstrated. The phenolic compounds exhibited an overall preference to the micelle-rich bottom phase for all the AMTPS investigated. A total of 60.1% total phenolic content with a DPPH free radical scavenging activity of 81.9% was recovered in the micelle-rich phase of the 10% (w/w) Pluronic L-61 AMTPS with a log partition coefficient, KTPC of 1.27. The AMTPS can serve as an alternative extraction technique for the recovery of various bioactive compounds from other plants.
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