Application of Antrodia camphorata Promotes Rat's Wound Healing In Vivo and Facilitates Fibroblast Cell Proliferation In Vitro.

Zahra A Amin,Hapipah M Ali,Mahmood A Abdulla,Pouya H Darvish,Mohammed A Alshawsh

doi:10.1155/2015/317693

Abstract

Antrodia camphorata is a parasitic fungus from Taiwan, it has been documented to possess a variety of pharmacological and biological activities. The present study was undertaken to evaluate the potential of Antrodia camphorata ethanol extract to accelerate the rate of wound healing closure and histology of wound area in experimental rats. The safety of Antrodia camphorata was determined in vivo by the acute toxicity test and in vitro by fibroblast cell proliferation assay. The scratch assay was used to evaluate the in vitro wound healing in fibroblast cells and the excision model of wound healing was tested in vivo using four groups of adult Sprague Dawley rats. Our results showed that wound treated with Antrodia camphorata extract and intrasite gel significantly accelerates the rate of wound healing closure than those treated with the vehicle. Wounds dressed with Antrodia camphorata extract showed remarkably less scar width at wound closure and granulation tissue contained less inflammatory cell and more fibroblast compared to wounds treated with the vehicle. Masson's trichrom stain showed granulation tissue containing more collagen and less inflammatory cell in Antrodia camphorata treated wounds. In conclusion, Antrodia camphorata extract significantly enhanced the rate of the wound enclosure in rats and promotes the in vitro healing through fibroblast cell proliferation.

Highlights

A wound can be described as the disruption of the typical anatomical shape and physiological function of a tissue
Wounds dressed with Antrodia camphorata or Intrasite gel showed considerable signs of dermal healing and healed faster than
Throughout the experiment, the percentage of healing in the vehicle control group wounds was significantly lower than those of Antrodia camphorata extract-treated groups and Intrasite gel wounds (Figure 1 and Table 1). In both stains (H&E and Masson’s trichrome) histology of wound area at day 14 after surgery showed that wound dressed with Antrodia camphorata extract showed comparatively less scar width at wound closure compared to the vehicle treated group (Figures 2, 3, 4, and 5), and the granulation tissue of wound area contained remarkably few inflammatory cells and more collagen and proliferating blood capillaries compared with vehicle treated group

Summary

Introduction

A wound can be described as the disruption of the typical anatomical shape and physiological function of a tissue. Wound healing process is a dynamic procedure which consists of three main stages including inflammation, proliferation, and tissue remodeling. The proliferation stage (active growth stage) includes tissue granulation and epithelialization, while the remodeling stage (reconstruction stage) involves maturation and remodeling of the scar tissue [1]. Natural products have been proven to enclose wound healing property and their primary or secondary metabolites have been shown to contribute potential repair mechanisms including activation of immune epithelial cells, cytokines, extracellular matrix (ECM), reactive oxygen species (ROS), growth factors, and different inflammatory mediators [2]. A huge number of medicinal mushrooms and plant extracts with wound healing potential have been reported by several authors [3,4,5,6]

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