Abstract
Adsorptive stripping voltammetry is a very sensitive and selective method for determination of drugs and organic substances in biological fluids. We have shown that determination of testosterone by adsorptive stripping voltammetry is possible using a lead film electrode. The lead film plating process and accumulation of testosterone were performed simultaneously from an acetate buffer solution of pH = 5.2 at a potential of -1.1 V. The measurements were carried out in undeaerated solutions. The detection limit was 9 x 10(-9) mol L(-1) for an accumulation time of 120 s; the relative standard deviation for 1 x 10(-7) mol L(-1) testosterone was 3.8%. The proposed voltammetric procedure for determination of testosterone could be applied to its determination in a pharmaceutical preparation and human urine samples directly without any separation steps.
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