Year-end Sale % OFF 
Abstract
An immunoperoxidase monolayer assay (IPMA) was adapted for the detection of antibodies to six arboviruses: three viruses within the flavivirus group (dengue 2, West Nile (WN) and yellow fever) and three in the phlebovirus group (Rift Valley fever (RVF), sandfly fever Naples and sandfly fever Sicilian). Antibody titers of homologoushyper-immune mouse ascitic fluid (HMAF) measured by IPMA were two to eight-fold less than those determined by ELISA. In tests with heterologous HMAF, cross-reactions frequently observed in ELISA, particularly in the flavivirusgroup, were absent in all IPMA titrations. With human serum samples tested for antibodies to RVF ( n = 52) and WN ( n = 90), the sensitivity of IPMA as compared with ELISA was 96 and 91%, respectively, specificity of IPMA was 100%. In addition, the IPMA format has several advantages that make it a useful alternative to ELISA for diagnosing arboviral infections under field conditions.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
