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Abstract
A modified enzyme-linked immunosorbent assay termed ELISA-elution assay was used as a screening tool to compare the efficiency of eluents for the dissociation of hen yolk immunoglobulin IgY–bovine IgG complexes. The potential denaturing effects of the eluents were also monitored. Different buffers (pH 2.3–7.5), containing various types and concentrations of salts (NaCl, (NH 4) 2SO 4 and MgCl 2) as well as polyols (ethylene glycol (EG) and glycerol) were compared to the commonly reported glycine·HCl (pH 2.8) buffer and to a commercially available eluent, Actisep™. Acidic pH buffers, Actisep™ and MgCl 2 (3.5 M with EG or 4 M without EG) all successfully dissociated IgY from immobilized IgG. However, some denaturation was apparent using MgCl 2 and, to a lesser extent, Actisep™. Furthermore, these same eluents demonstrated a diminished ability for liberating IgG from immobilized IgY IgG. Information on eluent efficacy obtained by the ELISA-elution assays was applied to selectively isolate lower affinity antibodies for immunoaffinity column chromatography.
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