Abstract
AbstractA novel electrophoretic method for detecting proteinase activity was used in order to study the column chromatographic separation of different acid, neutral and alkaline proteinases of a commercial crude proteinase preparation of Aspergillus oryzae (Rhozyme 41). Fractions obtained by gel filtration of Rhozyme 41 on Sephadex G‐100 were analyzed by the electrophoretic method and the radial diffusion method at pH 4.6 and pH 8.6. The electrophoretic method made it possible to discriminate between various acid, neutral and alkaline proteinases due to differences in the morphology of the clearing zones, whereas no differences in the morphology of the clearing zones could be observed when using the radial diffusion method.
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