Abstract
An aryl hydrocarbon receptor (AhR)-based, real-time polymerase chain reaction (PCR) assay (Procept®) was used to determine dioxin-like compounds in soil and sediment samples from seven sites known to be contaminated by dioxin and related compounds. The biological equivalent quotient (BEQ) generated by the Procept® assay was compared with toxicity equivalent (TEQ) measurements by isotope dilution gas chromatography-mass spectrometry (GC-MS). This research demonstrated that the Procept® assay has several limitations, when compared with GC-MS, including poorer detection limits and precision, and increased sensitivity to a wide range of AhR agonists and antagonists, such as polycyclic aromatic hydrocarbons (PAH) and polychlorinated biphenyls (PCBs). However, results indicate that the Procept® assay can be used as an effective screening technique for dioxin-like compounds at the EPA draft recommended interim preliminary remediation level for dioxin in soils of 72 pg/g TEQ.
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