Application of Amoxicillin in the pearl necklace test in the identification of the Anthraxic cause

Abstract

Identification of the causative agent of anthrax is an integral part of the diagnosis of the disease caused by it, which is registered annually on the territory of our country among animals and people. According to the guidelines for the diagnosis of the causative agent of anthrax, one of the identification tests is to study the sensitivity of the pathogen to penicillin with the registration of the phenomenon of the pearl necklace. Currently, penicillin salts are a hard-to-reach antibacterial drug. Based on this, we used amoxicillin, a penicillin-type antibiotic with a similar mechanism of action, which was the purpose of our study. A nutrient medium with an antibiotic was prepared by analogy with a medium with the addition of penicillin, according to MUC 4.2.2413-08. Amoxicillin in the form of amoxicillin trihydrate produced by Hemofarm (Serbia) was used as an antibacterial drug. To work, amoxicillin, by analogy with penicillin, was diluted 1,000,000 times with sterile Hottinger broth and introduced into a prepared nutrient medium. The loop of the grown daily culture of the strain K-STI-79 B. anthracis was seeded in 3 ml of the prepared medium and incubated for 3 hours at 37 ° C. During the incubation time, smears were prepared from the cells and stained according to the Rebiger. The smears were prepared after 15, 30 minutes and after 1, 2 and 3 hours. As a result of the work carried out, it was found that the use of amoxicillin in the identification of B. anthracis allows us to obtain the necessary results with a characteristic picture of a "pearl necklace". At the same time, the dynamics of changes in the morphology of cells subjected to incubation in a nutrient medium containing amoxicillin was studied. It was found that changes in the cell wall and cell shape can be observed after 15 minutes of incubation. After 1 hour, the cells underwent significant deformation with the appearance of a large number of separate spherical cell forms. After 2 hours of incubation, the chains of B. anthracis began to acquire the characteristic appearance of a pearl necklace, which was traced after 3 hours.