Application of a solid-phase fluorescence immunoassay to determine amoxicillin residues in fish tissue

Abstract

The present study demonstrates an application of Parallux (a solid-phase fluorescence immunoassay) for amoxicillin analysis in fish tissue. Amoxicillin at the recommended therapeutic dose (400 mg/kg body weight) was orally administered to three groups of 25 olive flounder ( Paralichthys olivaceus ), 25 rockfish ( Sebastes schlegeli ) and 25 red sea bream ( Pagrus major ) for 7 consecutive days. Amoxicillin was detected in the muscle of fish treated by the 3rd day of the withdrawal period. The recovery rates of all spiked muscle samples were > 86% of the spiked values. The present study showed that solid-phase fluorescence immunoassay can be easily adopted in predicting amoxicillin residues in the muscle tissue of farmed fish.