Abstract
The development of a homologous radioimmunoassay (RIA) for gilthead sea bream growth hormone (sbGH) is described. RIA sensitivity was 0.4 ng/mL, and ED50 was 1.74 ± 0.05 ng/mL. Intra- and inter-assay coefficients of variation were 3.4 and 8.8%, respectively, at ED50 levels. Mammalian growth hormones (GH), carp gonadotropin, chinook salmon gonadotropin, ovine porlactin, and recombinant tilapia prolactin did not show cross-reactivity. Serial dilutions of recombinant trout GH indicated a low but significant cross-reactivity. The displacement curves for plasma and pituitary homogenates from sparid fish (gilthead sea bream, blacktail, white sea bream, Couch's sea bream, and marmor-brassen) were parallel to that of the sbGH standard. Pituitary homogenates from other perciform fish tested (gaper, grey mullet, red mullet, and sea pike) also showed parallel slopes of inhibition. Pituitary homogenates from common sole, sea scorpion, forked hake, goldfish, rainbow trout, and European eel showed low or negligible cross-reactivity. These results suggest that the GHs of perciform fish have a number of similarities in structure. RIA of sea bream GH can be used to quantify the GH of sparid fish and perciform fish, provided a validation has been carried out.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
