Abstract
Currently, only few glufosinate-resistant genes are available for commercial application. Thus, developing novel glufosinate-resistant genes with commercial feasibility is extremely important and urgent for agricultural production. In this study, we transferred a newly isolated RePAT gene into a japonica rice variety Zhonghua11, resulting in a large number of independent T0 transgenic plants, most of which grew normally under high-concentration glufosinate treatment. Four transgenic plants with one intact RePAT expression cassette integrated into the intergenic region were selected. Agronomic performances of their T2 progenies were investigated, and the results suggested that the expression of RePAT had no adverse effect on the agronomic performance. Definite glufosinate resistance of the selected transgenic plants was further confirmed to be related to the expression of RePAT by assay on the medium and qRT-PCR. The inheritance and expression of RePAT in two transgenic plants were confirmed to be stable. Finally, the two-year field assay of glufosinate resistance suggested that the agronomic performance of the transgenic plant (PAT11) was not affected by high dosage of glufosinate (5000 g/ha). Collectively, our study proves the high resistance of a novel gene RePAT to glufosinate and provides a glufosiante-resistant rice variety with agricultural application potential.
Highlights
L-phosphinothricin (L-PPT) is the residue of a natural antibiotic bialaphos, which was found in the microbes Streptomyces viridochromogenes and Streptomyces hygroscopicus in the 1970s1
The final results showed that the applied glufosinate dosage was as high as 5000 g/ha, the agronomic traits of the transgenic rice expressing RePAT were not affected, indicating that RePAT is a valuable gene in developing glufosinate-resistant crops and the transgenic rice in this study is a good candidate for commercial production
As more than 70% of the independent T0 transgenic plants with RePAT grew normally without chlorosis (Fig. S1), it is reasonable to infer that the high glufosinate resistance exhibited by these transgenic plants was conferred by RePAT
Summary
L-phosphinothricin (L-PPT) is the residue of a natural antibiotic bialaphos, which was found in the microbes Streptomyces viridochromogenes and Streptomyces hygroscopicus in the 1970s1. It was found that different rice varieties expressing bar showed different levels of glufosinate resistance[11], which was reported in transgenic barley with bar[15] Some researchers attribute this phenomenon to the differences in genetic backgrounds of the recipient plants. To verify the potential of RePAT in developing glufosinate-resistant transgenic crops, in this study, we optimized the native nucleotide acid sequence of RePAT according to codon bias in rice and transferred RePAT into a japonica rice variety Zhonghua[11] by Agrobacterium-mediated transformation. The final results showed that the applied glufosinate dosage was as high as 5000 g/ha (corresponding to 10 times of the recommended glufosinate dosage for agricultural application), the agronomic traits of the transgenic rice expressing RePAT were not affected, indicating that RePAT is a valuable gene in developing glufosinate-resistant crops and the transgenic rice in this study is a good candidate for commercial production
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