Abstract
For over 100 years, field studies on arboviruses and the subsequent delivery and administration of live attenuated vaccines have been complicated by the need to maintain a so-called "cold chain," which is the source to destination refrigeration of biological materials. In this study we describe the application of a nonpaper based matrix and demonstrate preservation of chikungunya virus infectivity at ambient temperature for 7 days. The technique was successfully employed using infectious cell culture medium and infected mosquito homogenate samples. This technique provides a simple solution for conducting studies in resource-limited areas, where the maintenance of a cold chain is technically challenging.
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