Application of a New Self-Cleaning Filter for Colored Wastewaters Treatment Using Laccase Enzyme Immobilized on Activated CARBON powder and fiber

Abstract

The objective of this work is investigation of the adsorption and decomposition of Reactive Blue 19 from industrial wastewaters using laccase enzyme immobilized on activated carbon powder and fiber as adsorbent. Time, pH, temperature, stirring rate, the amount of the adsorbent, dye initial concentration, solution flow rate in the column and column height were studied as key operating parameters to determine the optimal adsorption conditions. The results indicated 80.70% of the adsorption for reactive blue 19 by immobilized laccase enzyme on adsorbent. In addition, kinetic parameters of the enzyme (Vmax and Km), optimal temperature effect, optimal pH and thermal stability of the free and stabilized laccase were studied and the results showed 66.66% of laccase enzyme immobilization yield on the adsorbent. The study of adsorption isotherms (Langmuir, Freundlich and Temkin) showed that the process follows a Langmuir model with a correlation coefficient (R2: 0.9389). Dye removal efficiency and characterization of the intermediate products of removal process were investigated using UV-Vis, TOC and LC/MS methods. Kinetic and thermodynamic models were studied based on the obtained results, the adsorption process follows a pseudo second order kinetic model. Determination of Gibbs free energy, ∆G, enthalpy, ∆H and entropy, ∆S, showed that the reaction is a spontaneous, exothermic process.