Abstract
A laboratory biofilm reactor (LBR) was modified to a new loop-type closed system in order to evaluate novel stents and catheter materials using 3D optical microscopy and Raman spectroscopy. Two metallic specimens, pure nickel and cupronickel (80% Cu-20% Ni), along with two polymers, silicone and polyurethane, were chosen as examples to ratify the system. Each set of specimens was assigned to the LBR using either tap water or an NB (Nutrient broth based on peptone from animal foods and beef extract mainly)—cultured solution with E-coli formed over 48–72 h. The specimens were then analyzed using Raman Spectroscopy. 3D optical microscopy was employed to corroborate the Raman Spectroscopy results for only the metallic specimens since the inherent roughness of the polymer specimens made such measurements difficult. The findings suggest that the closed loop-type LBR together with Raman spectroscopy analysis is a useful method for evaluating biomaterials as a potential urinary system.
Highlights
Biomaterials have been investigated and developed with regards to mechanical/material engineering functionalities [1] leading to the structural advancement of many materials
The study pays particular attention to the production and been evaluated by crystal violet staining, 3D optical microscopy, SEM-EDX, evaluation of biofilms pertaining to the urinary system, and potential materials for catheters and
Observations not provide data, the results suggest that copper in the cupronickel substrate worked to control the bacterial growth on the specimens’ surfaces, leading to the inhibition of biofilm formation to some extent
Summary
Biomaterials have been investigated and developed with regards to mechanical/material engineering functionalities [1] leading to the structural advancement of many materials. Biofilm formation on materials, evaluation system has been lacking from the viewpoint of materials not from biological viewpoint,suitable but from a materials sciencetoand engineering perspective This aresearch investigates analytical methods study biofilm formation on materials, system combines both the biofilm formation process and its evaluation. The authors have designed not from a biological viewpoint, but from a materials science and engineering perspective Any such and produced a loop-type circulation laboratory biofilm reactor (LBR). The study pays particular attention to the production and been evaluated by crystal violet staining, 3D optical microscopy, SEM-EDX (lower vacuum condition), evaluation of biofilms pertaining to the urinary system, and potential materials for catheters and. The LBR system to was to workand outside of a materials human body and investigations evaluation of biofilms pertaining themodified urinary system, potential for catheters and stents. Artificial and accelerated production of biofilm, including their evaluation by analytical methods
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