Abstract
Parallel plate flow chambers are widely used to expose cultured cells to physiological flows for the investigation of a variety of diseases. These applications usually involve the generation of continuous and steady fluid flow over cell monolayers for extended durations, usually a few days. Another technique is to generate a fast high-stress wave over the cells to see the immediate effect of flow-induced stresses. This can be achieved by propagating an air/liquid interface, in other words, a bubble, over cell monolayers. The approach is relevant to the reopening event of fluid-filled lung bronchioles and alveoli during mechanical ventilation therapy of Acute Respiratory Distress Syndrome. This article explains how we generate a stress wave using a parallel plate flow chamber and presents representative results of this wave on cultured lung epithelial cells.
Highlights
Cells in the human body are constantly exposed to physical forces [1]
These diseases are studied in vitro by exposing cultured cells to flow-induced stresses. This can be achieved by the use of parallel plate flow chambers, in which cultured cells in the bottom coverslip are exposed to fluid flow
We present our protocol for the generation of a plate chamber is composed of aon bottom cover slip,The an parallel upper coverslip, andisacomposed silicone membrane bubble flow-induced stress wave cell monolayers
Summary
Cells in the human body are constantly exposed to physical forces [1]. While physiological levels of these stresses are important in proper cell functioning, abnormal levels were shown to implicate the progression of diseases due to cellular injuries [4,5]. In most cases, these diseases are studied in vitro by exposing cultured cells to flow-induced stresses. These diseases are studied in vitro by exposing cultured cells to flow-induced stresses This can be achieved by the use of parallel plate flow chambers, in which cultured cells in the bottom coverslip are exposed to fluid flow. This high-stress wave can be generated by propagating an air/liquid interface, in other words, an air bubble, over cell monolayers
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