Application of a benchtop colorimetric method for quantification of blood propofol levels.

Abstract

Quantification of plasma propofol (2,6-diisopropylphenol) in the context of clinical anaesthesia is challenging because of the need for offline blood sample processing using specialised laboratory equipment and techniques. In this study we sought to refine a simple procedure using solid phase extraction and colorimetric analysis into a benchtop protocol for accurate blood propofol measurement.The colorimetric method based on the reaction of phenols (e.g. propofol) with Gibbs reagent was first tested in 10% methanol samples (n = 50) containing 0.5-6.0µg/mL propofol. Subsequently, whole blood samples (n = 15) were spiked to known propofol concentrations and processed using reverse phase solid phase extraction (SPE) and colorimetric analysis.The standard deviation of the difference between known and measured propofol concentrations in the methanol samples was 0.11µg/mL, with limits of agreement of - 0.21 to 0.22µg/mL. For the blood-processed samples, the standard deviation of the difference between known and measured propofol concentrations was 0.09µg/mL, with limits of agreement - 0.18 to 0.17µg/mL.Quantification of plasma propofol with an error of less than 0.2µg/mL is achievable with a simple and inexpensive benchtop method.